Biología de procariotas y gametas
Lucía Zalazar, Pablo Pagola, María Victoria Miró, María Sandra Churio, Micaela Cerletti, María Celeste Martínez, María Iniesta-Cuerda, Ana Josefa Soler Valls, Andreina Cesari*, Rosana De Castro* (*corresponding authors) Bacterioruberin extracts from a hyperpigmented Haloferax volcanii strain: Antioxidant activity and bioactive properties on sperm cells J. Appl. Microbiol. 126, 769-810 2018 doi: 10.1111/jam.14160.
Micaela Cerletti*, María I. Giménez, Christian Troetschel, Celeste D’Alessandro, Ansgar Poetsch, Rosana E. De Castro, Roberto A. Paggi* (*corresponding authors) Proteomic study of the exponential-stationary growth phase transition in the haloarchaea Natrialba magadii and Haloferax volcanii Proteomics, Dataset Brief 1800116 (1-4) 2018 doi: 10.1002/pmic.201800116
Alyne Marem, Lilian C.G. Oliveira, Débora N. Okamoto, Diego M. Ruiz, Roberto A. Paggi, Marcia Y. Kondo, Iuri E. Gouvea Dr, Maria A. Juliano, Rosana E. De Castro, Luiz Juliano, Marcelo Icimoto Functional roles of C-terminal extension (CTE) of salt-dependent peptidase activity of the Natrialba magadii extracellular protease (NEP) Int. J.Biol. Macromol. 113, 1134-1141. 2018 doi: 10.1016/j.ijbiomac.2018.03.026
Micaela Cerletti, Roberto Paggi, Christian Troetschel, Maria Celeste Ferrari, Carina Ramallo Guevara, Stefan Albaum, Ansgar Poetsch*, Rosana De Castro* (*corresponding authors) LonB Protease is a Novel Regulator of Carotenogenesis Controlling Degradation of Phytoene Synthase in Haloferax volcanii. J. Proteome Res. 17, 1158-1171 2018 doi: 10.1021/acs.jproteome.7b00809
Mariana I. Costa, Micaela Cerletti, Roberto A. Paggi, Christian Trötschel, Rosana E. De Castro, Ansgar Poetsch* and María I. Giménez* (*corresponding authors) Haloferax volcanii Proteome Response to Deletion of a Rhomboid Protease Gene J. Proteome Res.2018, 17, 961-977 2018 doi: 10.1021/acs.jproteome.7b00530
Gustavo Frattini, Yamila Pirosanto, Mayra Gómez Vitolo, Andreina Cesari, Pablo Ezequiel Meretta, Alicia Pené Evaluation of the Efficacy of the Laboratories of Embryology and Pathological Anatomy in the Analysis of Testicular Biopsies of Patients with Azoospermia Revista Argentina de Urología 83 (3): 96-100 2018  
Yamila Pirosanto, Gustavo Frattini, Andreina Césari, Pablo Ezequiel Meretta, Alicia Pené Evaluación de parámetros con valor predictivo para la recuperación espermática en la biopsia testicular de pacientes con azoospermia. Reproducción (SAMER) 33(2):09-16 2018  
Buchelly Imbachí F, Zalazar L, Pastore JI, Greco MB, Iniesta Cuerda M, Garde JJ, Soler AJ, Ballarin V, Cesari A Objective evaluation of ram and buck sperm motility by using novel sperm tracker software Reproduction 156(1):11-21 2018 doi: 10.1530/REP-17-07555
Corti Monzón,G, Nisenbaum,M, Herrera Seitz, M.K., Murialdo S.E New findings on aromatic compounds degradation and their metabolic pathways, the biosurfactant production and motility of the halophilic bacterium Halomonas sp. KHS3 Current Microbiology, 75(8):1108-1118. 2018 doi: 10.1007/s00284-018-1497-x
Gasperotti, Ana Florencia; María Victoria Revuelta; Claudia Alicia Studdert; María Karina Herrera Seitz Identification of two different chemosensory pathways in representatives of the genus Halomonas., BMC Genomics 19:266. 2018 doi: 10.1186/s12864-018-4655-4
Asaro, A, Paggi, R.A., del Valle, J.C., Lopez Mañanes, A. Glucose homeostasis in the euryhaline crab Cytograpsus angulatus: effects of the salinity in the amylase, maltase and sucrase activities in the hepatopancreas and in the carbohydrate reserves in different tissues. Comp Biochem Physiol, Part B 216 (1): 39-47. 2018 doi: 10.1016/j.cbpb.2017.11.012
Giraldo Mejía, H. F., Lucia Yohai, Andrea Pedetta, Karina Herrera Seitz, Raúl Ariel Procaccini, Sergio Antonio Pellice Epoxy-silica/clay nanocomposite for silver-based antibacterial thin coatings: Synthesis and structural characterization Journal of Colloid and Interface Science 508 332–341. 2017 doi: 10.1016/j.jcis.2017.08.058
Pedetta A, Massazza DA, Herrera Seitz MK, Studdert CA. Mutational replacements at the "glycine hinge" of the E. coli chemoreceptor Tsr support a signaling role for the C-helix residue. Biochemistry. 19;56(50):6626. 2017 doi: 10.1021/acs.biochem.7b00455
Ledesma A, Zalazar L, Fernández-Alegre E, Cesari A, Hozbor F, Martinez-Pastor F. Seminal plasma proteins modify the distribution of sperm subpopulations in cryopreserved semen of rams with lesser fertility. Anim Reprod Sci. 184:44-50 2017 doi: 10.1016/j.anireprosci.2017.06.015.
Alonso, C; Osycka-Salut, C; Castellano, L; Cesari, A; Di Siervi, N; Mutto, A; Johannisson, A; Morrell, J; Davio, C; Perez-Martinez, S. Extracellular cAMP Activates Molecular Signalling Pathways Associated to Sperm Capacitation in Bovines. Mol Hum Reprod. ;23(8):521-534 2017 doi: 10.1093/molehr/gax030
Antonela Asaro, Roberto Paggi, Rosana De Castro, Alejandra López Mañanes Amylase in the hepatopancreas of an euryhaline burrowing crab: characteristics and modulation Turk J Zool. 41:443-453. 2017 doi: 10.3906/zoo-1601-10.
Di Meglio L, Santos F, Gomariz, M, Almansa, C, López C, Antón-Botella J and Nercessian D “Seasonal dynamics of extremely halophilic microbial communities in three Argentinian salterns” FEMS Microbiol Ecol. 92 (12): fiw184. 2016 doi:
Ledesma A, Fernández-Alegre E, Cano A, Hozbor F, Martínez-Pastor F, Cesari A Seminal plasma proteins interacting with sperm surface revert capacitation indicators in frozen-thawed ram sperm. Anim Reprod Sci. 173:35-41 2016 doi: 10.1016/j.anireprosci.2016.08.007.
Zalazar L, Ledesma A, Hozbor F, Cesari A. Heterologous recombinant protein with decapacitating activity prevents and reverts cryodamage in ram sperm: An emerging biotechnological tool for cryobiology. Anim Reprod Sci.164:31-9 2016 doi: 10.1016/j.anireprosci.2015.11.007
A. Ledesma, J. Manes, G. Ríos, J. Aller, A. Cesari, R. Alberio, F. Hozbor Effect of seminal plasma on post-thaw quality and functionality of Corriedale ram sperm obtained by electroejaculation and artificial vagina. Reprod Domest Anim.50(3):386-92 2015 doi: 10.1111/rda.
Ríos G, Buschiazzo J, Mucci NC, Kaiser GG, Cesari A, Alberio RH. Combined EGF and Hyaluronic acid supplementation of in vitro maturation medium and its impact on bovine oocyte proteome and competence. Theriogenology 83(5):874-80 2015 doi: 10.1016/j.theriogenology.2014.11.022
Débora Nercessian, Leonardo Dimeglio, Rosana De Castro, Roberto Paggi. Exploring the multiple biotechnological potential of halophilic microorganisms isolated from two Argentinean salterns. Extremophiles 19: 1133-1143 2015 doi: 10.1007/s00792-015-0785-7
Micaela Cerletti, Roberto A. Paggi, Carina Ramallo Guevara, Ansgar Poetsch* and Rosana E. De Castro* ( *Corresponding authors) Data in support of global role of the membrane protease LonB in Archaea: Potential protease targets revealed by quantitative proteome analysis of a lonB mutant in Haloferax volcanii Data in Brief (2015) pp. 50-53 2015 doi: 10.1016/j.dib.2015.04.013
Micaela Cerletti, Roberto A. Paggi, Carina Ramallo Guevara, Ansgar Poetsch* and Rosana E. De Castro* ( *Corresponding authors). Global role of the membrane protease LonB in Archaea: Potential protease targets revealed by quantitative proteome analysis of a lon mutant in Haloferax volcanii J. Proteomics 121, 1-14 2015 doi: 10.1016/j.jprot.2015.03.016
María Inés Giménez*, Micaela Cerletti and Rosana Esther De Castro* (*corresponding authors) Archaeal membrane-associated proteases: insights on Haloferax volcanii and other haloarchaea Front. Microbiol. (6) art 39:1-9. In: “Archaeal cell envelope and surface structures”. Edited by S.V Albers and M. Pohlschroder. 2015 doi: 10.3389/978-2-88919-773-6
Ana Florencia Gasperotti, Claudia Alicia Studdert, Santiago Revale, María Karina Herrera Seitz Draft Genome Sequence of Halomonas sp. KHS3, a Polyaromatic Hydrocarbon-Chemotactic Strain Genome Announc., ASM, vol. 3 no. 2 e00020-15. 2015 doi: 10.1128/genomeA.00020-15.
Herrera Seitz, K, Frank, V, Massazza, DA, Vaknin, A and Studdert, CA Bacterial chemoreceptors of different length classes signal independently. Mol Microbiol. 93(4):814-22. 2014 doi: 10.1111/mmi.12700
Ledesma A., J. Manes, A. Cesari, R. Alberio, F. Hozbor. Electroejaculation increases low molecular weight proteins in seminal plasma modifying sperm quality in Corriedale rams. Reprod Domest Anim. 49(2):324-32 2014 doi: 10.1111/rda.12279
Roberto A. Paggi. Maria I., Giménez, Rosana E. De Castro and Andreina Cesari A simple technique to improve the resolution of membrane acidic proteins of the haloarchaeon Haloferax volcanii by 2D electrophoresis. Electrophoresis 35, 3518-3522 2014 doi: 10.1002/elps.201400407
Di Meglio L, Busalmen JP, Pastore JI, Ballarín VL and Nercessian D Hyperhalophilic archaeal biofilms: growth kinetics, structure and antagonic interaction in continuous culture Biofouling 30 (2): 237-245 2014 doi: 10.1080/08927014.2013.860136
J. Parente, A. Casabuono, M.C. Ferrari, R.A. Paggi, R.E. De Castro, A. Couto and M.I. Giménez A Rhomboid Protease Gene Deletion Affects a Novel Oligosaccharide N-linked to the S-layer glycoprotein of Haloferax volcanii. J. Biol Chem. 289(16):11304-17 2014 doi: 10.1074/jbc.M113.546531
Micaela Cerletti, María J. Martínez, María I. Giménez, Diego E. Sastre, Roberto A. Paggi and Rosana E. De Castro. The LonB protease controls membrane lipids composition and is essential for viability in the extremophilic haloarchaeon Haloferax volcanii Environ. Microbiol. 16 (6): 1779-1792 2014 doi: 10.1111/1462-2920.12385.
L. Zalazar,C. A. I. Alonso, R. E. De Castro, A. Cesari. An alternative easy method for antibody purification and protein-protein interactions analyzes using GST fusion proteins immobilized onto glutathione agarose. Anal Bioanal Chem 2014 doi: 10.1007/s00216-013-7533-6.
D. Assis, L. Zalazar, M. Juliano, R. E. De Castro and A. Cesari Novel inhibitory activity for serine protease inhibitor Kazal type-3 (SPINK3) on human recombinant kallikreins Prot. Pept. Lett. 20 (10): 1098-1107 (2013). 2013 doi: 10.2174/0929866511320100003
Herrera Seitz, K; Soto, D and Studdert, C “A chemoreceptor from Pseudomonas putida forms active signaling complexes in E. coli”. Microbiology (SGM), (158), 2283-2292 2012 doi: 10.1099/mic.0.059899-0
Sofia Österberg, Anna Åberg, M Karina Herrera Seitz, Magnus Wolf-Watz and Victoria Shingler Genetic dissection of a motility-associated c-di-GMP signalling protein of Pseudomonas putida Environmental Microbiology Reports. Volume 5, Issue 4, pages 556–565 2012 doi: 10.1111/1758-2229.12045
Massazza, DA, Silvina A. Izzo, Florencia Gasperotti, M. Karina Herrera Seitz and Claudia A. Studdert Functional and Structural Effects of Seven-Aminoacid Deletions on the Coiled Coil Cytoplasmic Domain of a Chemoreceptor Molecular Microbiology, 83(1), 224–239 2012 doi: 10.1111/j.1365-2958.2011.07928.x
Diego M. Ruiz, Roberto A. Paggi, Maria I. Giménez and Rosana E. De Castro Autocatalytic maturation of the Tat-dependent halophilic subtilase Nep produced by the archaeon Natrialba magadii. J. Bacteriol.194 (14): 3700. 2012 doi: 10.1128/JB.06792-11
Shivakumara Siddaramappa, Jean F Challacombe, Rosana E De Castro, Friedhelm Pfeiffer, Diego E Sastre, María I Giménez, Roberto A Paggi, , John C Detter, Karen W Davenport, Lynne A Goodwin, Nikolaos Kyrpides, Roxanne Tapia, Samuel Pitluck, Susan Lucas, Tanja Woyke and Julie A Maupin-Furlow A comparative genomics perspective on the genetic content of the alkaliphilic haloarchaeon Natrialba magadii ATCC 43099 BMC Genomics 13:165-184 2012 doi: 10.1186/1471-2164-13-165
Enrique A. Madrid, Micaela Cerletti, Roberto A. Paggi, Diego E. Sastre and Rosana E. De Castro Identification of growth-dependent transcripts in the haloalkaliphilic archaeon, Natrialba magadii J. Gen.Appl. Microbiol. 58 (6): 53-57 2012 doi: 10.2323/jgam.58.53
Zalazar L., Clementi M., Lombardo C., Fornes MW, Lamattina L., De Castro R. and Cesari A SPINK3 (caltrin) modulates mouse sperm acrosome exocytosis but not capacitation through NO level reduction and independently of trypsin inhibitory activity Reproduction, 143:281-295 2012 doi: 10.1530/REP-11-0107.
Souza, T.A.C.B.; Okamoto, D.N.; Ruiz D.M.; Oliveira, LC.G; Kondo, M.Y.; Juliano, L; De Castro, R.E.; Gouvea, I.E. and Murakami, M.T. Correlation between catalysis and tertiary structure arrangement in an archaeal halophilic subtilase . Biochimie, 94:798-805 2012 doi: 10.1016/j.biochi.2011.11.011.
Ordóñez, MV; Nercessian, D and Conde, RD “Nmag_2608, an extracellular ubiquitin-like domain containing protein from the haloalkaliphilic archaeon Natrialba magadii. Extremophiles 16 (3): 437-446 2012 doi: 10.1007/s00792-012-0443-2
Lucía Zalazar, Marisa Clementi, Miguel W Fornes, Andreina Cesari Búsqueda de inhibidores de serina proteasas en glándulas accesorias del sistema reproductor masculino. Reproducción (SAMER) 27:79-89. 2012  
S. D’Ippolito, R. E. De Castro, M.K. Herrera Seitz. Chemotactic responses to gas oil of Halomonas spp strains isolated from saline environments in Argentina Revista Argentina de Microbiología 43: 107-110 (2011). 2011 doi: 10.1590/S0325-75412011000200007
Diego E. Sastre, Roberto A. Paggi and Rosana E. De Castro. The Lon protease from the haloalkaliphilic archaeon Natrialba magadii is transcriptionally linked to a cluster of putative membrane proteases and displays DNA-binding activity Microbiological Research. 166, 304-313 2011 doi: 10.1016/j.micres.2010.07.002
Bernardini A, Hozbor F, Sanchez E, Fornés MW, Alberio RH and Cesari A. Conserved ram seminal plasma proteins bind to the sperm membrane and repair cryopreservation damage. Theriogenology 76(3) pp. 436-447. 2011 doi:10.1016/ j. theriogenology. 2011.02.020
Ordóñez MV, Guillén J, Nercessian D, Villalaín J, Conde RD “Secondary structure determination by FTIR of an archaeal ubiquitin-like polypeptide from Natrialba magadii. Euro Biophys J. 40 (9): 1101-1107 2011 doi: 10.1007/s00249-011-0719-y
Diego M. Ruiz, Nancy Iannuci, Osvaldo Cascone and Rosana E. De Castro Peptide synthesis catalyzed by a haloalkaliphilic serine protease from the archaeon Natrialba magadii Lett. Appl. Microbiol. 51, 691-696. 2010 doi: 10.1111/j.1472-765X.2010.02955.x
R. A Paggi, E. A. Madrid, C.P. D’Alessandro, Cerletti, M. and R. E. De Castro Growth phase-dependent biosynthesis of NEP, a halolysin-like protease secreted by the haloalkaliphilic archaeon Natrialba magadii. Lett. Appl.Microbiol. 51: 36-41 2010 doi: 10.1111/j.1472-765X.2010.02955.x
D. Nercessian, F.B. Duville, M. Desimone, S. Simison and J.P. Busalmen “Metabolic turnover and catalase activity of biofilms of P. fluorescens (ATCC 17552) as related to copper corrosion Water Research, 44 (8):2592-2600 2010 doi: 10.1016/j.watres.2010.01.014
Cesari, A, Monclus MA, Tejón GP, Clementi M and Fornes MW. Regulated serine proteinase lytic system on mammalian sperm surface: there must be a role (Review) Theriogenology (2010) 74 (5) sep 2010, 699–711. 2010 doi: 10.1016/j.theriogenology.2010.03.029
Monclus MA, Andreina C, Cabrillana ME, Saez Lancellotti TE, Rensetti DE, Clementi MA, Boarelli PV, Vincenti AE, Fornés MW. Protein fraction isolated from epididymal fluid re-associates sperm in vitro: Possible role of serpins in rat Rosettes assembly. Molec Reprod Dev. 77(5):410-9. 2010 doi: 10.1002/mrd.21155.
Débora Nercessian, Cristina Marino-Buslje, María Victoria Ordóñez, Rosana E. De Castro and Rubén Danilo Conde Presence of structural homologs to ubiquitin in haloalkaliphilic Archaea Int. Microbiol.12:167-173 2009 10.2436/20.1501.01.95