AUTHORSTITLEJOURNALYEARDOI
Cerletti M, Rabino A, Paggi RA, Ferrari C, Poetsch A, Savilahti H, Kiljunen S, De Castro RE The C-terminal region of phytoene synthase is a key element to control carotenoid biosynthesis in the haloarchaeon Haloferax volcanii. Biochem J. 2022 doi: 10.1042/BCJ20220403
Rosana E. De Castro and Ansgar Poetsch EDITORIAL: Proteases of the Prokaryotic Cell Envelope Frontiers in Microbiology 2022

10.3389/fmicb.2022.988067

De Castro RE., Giménez MI., Cerletti M, Paggi RA., Costa MI. Proteolysis at the Archaeal Membrane: Advances on the Biological Function and Natural Targets of Membrane-Localized Proteases in Haloferax volcanii. Front Microbiol 2022 10.3389/fmicb.2022.940865
Nicolli AR, Alonso CAI, Otamendi C, Cerletti M, Poetsch A, Sharma V, Zalazar L, Perez-Martinez S, Cesari A. How, where and when is SPINK3 bound and removed from mouse sperm? Reproduction 2022 doi: 10.1530/REP-21-01
Buchelly Imbachí F, Zalazar L, Pastore JI, Nicolli A, Ledesma A,Hozbor F, Ballarin V, Cesari A. Clustering and classification software for sperm subpopulation analysis. Biomechanics and Biomedical Engineering: Imaging & Visualization 2022 doi: 10.1080/21681163.2021.2012831
Balmaceda, R, Ramos Ricciuti, F,  Redersdorff I, Veinticcinque, L, Studdert C and Herrera Seitz, K. Chemosensory pathways ofHalomonas titanicae KHS3 control chemotaxis behaviour and biofilm formation. Microbiology SGM 2022 doi: 10.1099/mic.0.001251
Ana F. Gasperotti, M. Karina Herrera Seitz, Rocío S. Balmaceda, Luciano M. Prosa, Kirsten Jung, Claudia A. Studdert Direct binding of benzoate derivatives to two chemoreceptors with Cache sensor domains in Halomonas titanicae KHS3 Mol Microbiol 2021 10.1111/mmi.14630
Perez-Martinez Silvina, Soler Ana Josefa, Cesari Andreina Editorial: Molecular and Cellular Physiology of Gametes in Domestic and Wild Animal Models Frontiers in Cell and Developmental Biology 2021 10.3389/fcell.2021.805036
Ledesma, A., Zalazar, L., Greco, M., Hozbor, F., Cesari, A. Recombinant TrxAFNIIx4His6 improves post-thaw motility of ram sperm measured by a sperm motility tracker software Tropical Animal Health and Production 2021  
Schulze S.; Z. Adams; M. Cerletti; R. De Castro; S. Ferreira-Cerca; C. Fufezan; M. I. Giménez; M. Hippler; Z. Jevtic; R. Knüppel; G. Legerme; C. Lenz; A. Marchfelder; J. Maupin-Furlow; R. A. Paggi; F. Pfeiffer; A. Poetsch; H. Urlaub; M. Pohlschroder. The Archaeal Proteome Project advances knowledge about archaeal cell biology through comprehensive proteomics. Nat. Commun 2020  
 Ferrari MC, Cerletti M, Paggi RA, Troetschel C, Poetsch A, De Castro RE. The LonB protease modulates the degradation of CetZ1 involved in rod-shape determination in Haloferax volcanii. J Proteomics 2020  doi: 10.1016/j.jprot.2019.103546
Zalazar Lucia, Stival Cintia, Nicolli Anabella R., De Blas Gerardo A., Krapf Dario, Cesari Andreina Male Decapacitation Factor SPINK3 Blocks Membrane Hyperpolarization and Calcium Entry in Mouse Sperm Frontiers in Cell and Developmental Biology 2020 10.3389/fcell.2020.575126
 M.I. Costa, M.S. Álvarez‐Cerimedo, D. Urquiza, M.A. Ayude, C.E. Hoppe, D.P. Fasce, R.E. De Castro, M.I. Giménez Synthesis, characterization and kinetic study of silver and gold nanoparticles produced by the archaeon Haloferax volcanii Journal of Applied Microbiology 2020  doi.org/10.1111/jam.14726
Patricia Peris-Frau, Alicia Martín-Maestro, María Iniesta-Cuerda, Irene Sánchez-Ajofrín, Andreina Cesari, J. Julián Garde, Margarita Villar, Ana J. Soler Cryopreservation of ram sperm alters the dynamic changes associated with in vitro capacitation Theriogenology 2020 10.1016/j.theriogenology.2020.01.046
Lucía Zalazar, María Iniesta-Cuerda, Irene Sánchez-Ajofrín, J. Julián Garde, Ana Josefa Soler Valls, Andreina Cesari Recombinant SPINK3 improves ram sperm quality and in vitro fertility after cryopreservation Theriogenology 2020 10.1016/j.theriogenology.2019.12.019.
Buchelly, F.J., Isa Jara, R.F., Zalazar, L., Cesari, A., Pastore, J.I., Ballarin, V.L. Comparative Analysis of Different Techniques to Determine Motility Parameters in Video Sequences of Ram and Buck Sperm IFMBE Proceedings 2020 10.1007/978-3-030-30648-9_50
Ramírez-Vasquez, R., Cesari, A., Greco, M.B., Cano, A., Hozbor, F. Extenders modify the seminal plasma ability to minimize freeze-thaw damage on ram sperm Reproduction in Domestic Animals 2019 10.1111/rda.13571
Ramírez-Vasquez, R.R.A., Cano, A., Hozbor, F.A., Cesari, A. Cryopreservation and egg yolk extender components modify the interaction between seminal plasma proteins and the sperm surface Theriogenology 2019  
 Alba Ledesma, Lucía Zalazar, Francisco Buchelly Imbachi, Juan Ignacio Pastore, Paula Brown, Edward Mitch Eddy, Federico Hozbor, Andreina Cesari Recombinant peptide reverses cryo-capacitation in ram sperm and improves in vitro fertilization  Animal Reproduction Science 2019  10.1016/j.anireprosci.2019.05.016.
 Ramírez-Vasquez, R., Cesari, A., Hozbor, F.  Components of the extender and seminal plasma can alter the response of ram spermatozoa to cryopreservation | Los componentes del diluyente y el plasma seminal pueden modificar la respuesta de los espermatozoides ovinos a la criopreservación Taurus 2019  
Lucía Zalazar, Pablo Pagola, María Victoria Miró, María Sandra Churio, Micaela Cerletti, María Celeste Martínez, María Iniesta-Cuerda, Ana Josefa Soler Valls, Andreina Cesari*, Rosana De Castro* (*corresponding authors) Bacterioruberin extracts from a hyperpigmented Haloferax volcanii strain: Antioxidant activity and bioactive properties on sperm cells J. Appl. Microbiol. 2019 doi: 10.1111/jam.14160.
Micaela Cerletti*, María I. Giménez, Christian Troetschel, Celeste D’Alessandro, Ansgar Poetsch, Rosana E. De Castro, Roberto A. Paggi* (*corresponding authors) Proteomic study of the exponential-stationary growth phase transition in the haloarchaea Natrialba magadii and Haloferax volcanii Proteomics 2018 doi: 10.1002/pmic.201800116
Alyne Marem, Lilian C.G. Oliveira, Débora N. Okamoto, Diego M. Ruiz, Roberto A. Paggi, Marcia Y. Kondo, Iuri E. Gouvea Dr, Maria A. Juliano, Rosana E. De Castro, Luiz Juliano, Marcelo Icimoto Functional roles of C-terminal extension (CTE) of salt-dependent peptidase activity of the Natrialba magadii extracellular protease (NEP) Int. J.Biol. Macromol. 2018 doi: 10.1016/j.ijbiomac.2018.03.026
Micaela Cerletti, Roberto Paggi, Christian Troetschel, Maria Celeste Ferrari, Carina Ramallo Guevara, Stefan Albaum, Ansgar Poetsch*, Rosana De Castro* (*corresponding authors) LonB Protease is a Novel Regulator of Carotenogenesis Controlling Degradation of Phytoene Synthase in Haloferax volcanii. J. Proteome Res. 2018 doi: 10.1021/acs.jproteome.7b00809
Mariana I. Costa, Micaela Cerletti, Roberto A. Paggi, Christian Trötschel, Rosana E. De Castro, Ansgar Poetsch* and María I. Giménez* (*corresponding authors) Haloferax volcanii Proteome Response to Deletion of a Rhomboid Protease Gene J. Proteome Res. 2018 doi: 10.1021/acs.jproteome.7b00530
Gustavo Frattini, Yamila Pirosanto, Mayra Gómez Vitolo, Andreina Cesari, Pablo Ezequiel Meretta, Alicia Pené Evaluation of the Efficacy of the Laboratories of Embryology and Pathological Anatomy in the Analysis of Testicular Biopsies of Patients with Azoospermia Revista Argentina de Urología  2018  
Yamila Pirosanto, Gustavo Frattini, Andreina Césari, Pablo Ezequiel Meretta, Alicia Pené Evaluación de parámetros con valor predictivo para la recuperación espermática en la biopsia testicular de pacientes con azoospermia. Reproducción
2018  
Buchelly Imbachí F, Zalazar L, Pastore JI, Greco MB, Iniesta Cuerda M, Garde JJ, Soler AJ, Ballarin V, Cesari A Objective evaluation of ram and buck sperm motility by using novel sperm tracker software Reproduction
2018 doi: 10.1530/REP-17-07555
Corti Monzón,G, Nisenbaum,M, Herrera Seitz, M.K., Murialdo S.E New findings on aromatic compounds degradation and their metabolic pathways, the biosurfactant production and motility of the halophilic bacterium Halomonas sp. KHS3 Current Microbiology 2018 doi: 10.1007/s00284-018-1497-x
Gasperotti, Ana Florencia; María Victoria Revuelta; Claudia Alicia Studdert; María Karina Herrera Seitz Identification of two different chemosensory pathways in representatives of the genus Halomonas., BMC Genomics 2018 doi: 10.1186/s12864-018-4655-4
Asaro, A, Paggi, R.A., del Valle, J.C., Lopez Mañanes, A. Glucose homeostasis in the euryhaline crab Cytograpsus angulatus: effects of the salinity in the amylase, maltase and sucrase activities in the hepatopancreas and in the carbohydrate reserves in different tissues. Comp Biochem Physiol 2018 doi: 10.1016/j.cbpb.2017.11.012
Giraldo Mejía, H. F., Lucia Yohai, Andrea Pedetta, Karina Herrera Seitz, Raúl Ariel Procaccini, Sergio Antonio Pellice Epoxy-silica/clay nanocomposite for silver-based antibacterial thin coatings: Synthesis and structural characterization Journal of Colloid and Interface Science 2017 doi: 10.1016/j.jcis.2017.08.058
Pedetta A, Massazza DA, Herrera Seitz MK, Studdert CA. Mutational replacements at the "glycine hinge" of the E. coli chemoreceptor Tsr support a signaling role for the C-helix residue. Biochemistry. 19;56(50):6626. 2017 doi: 10.1021/acs.biochem.7b00455
Ledesma A, Zalazar L, Fernández-Alegre E, Cesari A, Hozbor F, Martinez-Pastor F. Seminal plasma proteins modify the distribution of sperm subpopulations in cryopreserved semen of rams with lesser fertility. Anim Reprod Sci 2017 doi: 10.1016/j.anireprosci.2017.06.015.
Alonso, C; Osycka-Salut, C; Castellano, L; Cesari, A; Di Siervi, N; Mutto, A; Johannisson, A; Morrell, J; Davio, C; Perez-Martinez, S. Extracellular cAMP Activates Molecular Signalling Pathways Associated to Sperm Capacitation in Bovines. Mol Hum Reprod 2017 doi: 10.1093/molehr/gax030
Antonela Asaro, Roberto Paggi, Rosana De Castro, Alejandra López Mañanes Amylase in the hepatopancreas of an euryhaline burrowing crab: characteristics and modulation Turk J Zool. 2017 doi: 10.3906/zoo-1601-10.
Ledesma A, Fernández-Alegre E, Cano A, Hozbor F, Martínez-Pastor F, Cesari A Seminal plasma proteins interacting with sperm surface revert capacitation indicators in frozen-thawed ram sperm. Anim Reprod Sci. 2016 doi: 10.1016/j.anireprosci.2016.08.007.
Zalazar L, Ledesma A, Hozbor F, Cesari A. Heterologous recombinant protein with decapacitating activity prevents and reverts cryodamage in ram sperm: An emerging biotechnological tool for cryobiology. Anim Reprod Sci. 2016 doi: 10.1016/j.anireprosci.2015.11.007
A. Ledesma, J. Manes, G. Ríos, J. Aller, A. Cesari, R. Alberio, F. Hozbor Effect of seminal plasma on post-thaw quality and functionality of Corriedale ram sperm obtained by electroejaculation and artificial vagina. Reprod Domest Anim 2015 doi: 10.1111/rda.
Ríos G, Buschiazzo J, Mucci NC, Kaiser GG, Cesari A, Alberio RH. Combined EGF and Hyaluronic acid supplementation of in vitro maturation medium and its impact on bovine oocyte proteome and competence. Theriogenology
2015 doi: 10.1016/j.theriogenology.2014.11.022
Débora Nercessian, Leonardo Dimeglio, Rosana De Castro, Roberto Paggi. Exploring the multiple biotechnological potential of halophilic microorganisms isolated from two Argentinean salterns. Extremophiles 2015 doi: 10.1007/s00792-015-0785-7
Micaela Cerletti, Roberto A. Paggi, Carina Ramallo Guevara, Ansgar Poetsch* and Rosana E. De Castro* ( *Corresponding authors) Data in support of global role of the membrane protease LonB in Archaea: Potential protease targets revealed by quantitative proteome analysis of a lonB mutant in Haloferax volcanii Data in Brief 2015 doi: 10.1016/j.dib.2015.04.013
Micaela Cerletti, Roberto A. Paggi, Carina Ramallo Guevara, Ansgar Poetsch* and Rosana E. De Castro* ( *Corresponding authors). Global role of the membrane protease LonB in Archaea: Potential protease targets revealed by quantitative proteome analysis of a lon mutant in Haloferax volcanii J. Proteomics 2015 doi: 10.1016/j.jprot.2015.03.016
María Inés Giménez*, Micaela Cerletti and Rosana Esther De Castro* (*corresponding authors) Archaeal membrane-associated proteases: insights on Haloferax volcanii and other haloarchaea Front. Microbiol. 2015 doi: 10.3389/978-2-88919-773-6
Ana Florencia Gasperotti, Claudia Alicia Studdert, Santiago Revale, María Karina Herrera Seitz Draft Genome Sequence of Halomonas sp. KHS3, a Polyaromatic Hydrocarbon-Chemotactic Strain Genome Announc. ASM 2015 doi: 10.1128/genomeA.00020-15.
Herrera Seitz, K, Frank, V, Massazza, DA, Vaknin, A and Studdert, CA Bacterial chemoreceptors of different length classes signal independently. Mol Microbiol 2014 doi: 10.1111/mmi.12700
Ledesma A., J. Manes, A. Cesari, R. Alberio, F. Hozbor. Electroejaculation increases low molecular weight proteins in seminal plasma modifying sperm quality in Corriedale rams. Reprod Domest Anim 2014 doi: 10.1111/rda.12279
Roberto A. Paggi. Maria I., Giménez, Rosana E. De Castro and Andreina Cesari A simple technique to improve the resolution of membrane acidic proteins of the haloarchaeon Haloferax volcanii by 2D electrophoresis. Electrophoresis 2014 doi: 10.1002/elps.201400407
J. Parente, A. Casabuono, M.C. Ferrari, R.A. Paggi, R.E. De Castro, A. Couto and M.I. Giménez A Rhomboid Protease Gene Deletion Affects a Novel Oligosaccharide N-linked to the S-layer glycoprotein of Haloferax volcanii. J. Biol Chem. 2014 doi: 10.1074/jbc.M113.546531
Micaela Cerletti, María J. Martínez, María I. Giménez, Diego E. Sastre, Roberto A. Paggi and Rosana E. De Castro. The LonB protease controls membrane lipids composition and is essential for viability in the extremophilic haloarchaeon Haloferax volcanii Environ. Microbiol. 2014 doi: 10.1111/1462-2920.12385.
L. Zalazar,C. A. I. Alonso, R. E. De Castro, A. Cesari. An alternative easy method for antibody purification and protein-protein interactions analyzes using GST fusion proteins immobilized onto glutathione agarose. Anal Bioanal Chem 2014 doi: 10.1007/s00216-013-7533-6.
D. Assis, L. Zalazar, M. Juliano, R. E. De Castro and A. Cesari Novel inhibitory activity for serine protease inhibitor Kazal type-3 (SPINK3) on human recombinant kallikreins Prot. Pept. Lett. 2013 doi: 10.2174/0929866511320100003
Herrera Seitz, K; Soto, D and Studdert, C A chemoreceptor from Pseudomonas putida forms active signaling complexes in E. coli Microbiology (SGM) 2012 doi: 10.1099/mic.0.059899-0
Sofia Österberg, Anna Åberg, M Karina Herrera Seitz, Magnus Wolf-Watz and Victoria Shingler Genetic dissection of a motility-associated c-di-GMP signalling protein of Pseudomonas putida Environmental Microbiology Reports. 2012 doi: 10.1111/1758-2229.12045
Massazza, DA, Silvina A. Izzo, Florencia Gasperotti, M. Karina Herrera Seitz and Claudia A. Studdert Functional and Structural Effects of Seven-Aminoacid Deletions on the Coiled Coil Cytoplasmic Domain of a Chemoreceptor Molecular Microbiology, 2012 doi: 10.1111/j.1365-2958.2011.07928.x
Diego M. Ruiz, Roberto A. Paggi, Maria I. Giménez and Rosana E. De Castro Autocatalytic maturation of the Tat-dependent halophilic subtilase Nep produced by the archaeon Natrialba magadii. J. Bacteriol. 2012 doi: 10.1128/JB.06792-11
Shivakumara Siddaramappa, Jean F Challacombe, Rosana E De Castro, Friedhelm Pfeiffer, Diego E Sastre, María I Giménez, Roberto A Paggi, , John C Detter, Karen W Davenport, Lynne A Goodwin, Nikolaos Kyrpides, Roxanne Tapia, Samuel Pitluck, Susan Lucas, Tanja Woyke and Julie A Maupin-Furlow A comparative genomics perspective on the genetic content of the alkaliphilic haloarchaeon Natrialba magadii ATCC 43099 BMC Genomics
2012 doi: 10.1186/1471-2164-13-165
Enrique A. Madrid, Micaela Cerletti, Roberto A. Paggi, Diego E. Sastre and Rosana E. De Castro Identification of growth-dependent transcripts in the haloalkaliphilic archaeon, Natrialba magadii J. Gen.Appl. Microbiol. 2012 doi: 10.2323/jgam.58.53
Zalazar L., Clementi M., Lombardo C., Fornes MW, Lamattina L., De Castro R. and Cesari A SPINK3 (caltrin) modulates mouse sperm acrosome exocytosis but not capacitation through NO level reduction and independently of trypsin inhibitory activity Reproduction 2012 doi: 10.1530/REP-11-0107.
Souza, T.A.C.B.; Okamoto, D.N.; Ruiz D.M.; Oliveira, LC.G; Kondo, M.Y.; Juliano, L; De Castro, R.E.; Gouvea, I.E. and Murakami, M.T. Correlation between catalysis and tertiary structure arrangement in an archaeal halophilic subtilase . Biochimie 2012 doi: 10.1016/j.biochi.2011.11.011.
Lucía Zalazar, Marisa Clementi, Miguel W Fornes, Andreina Cesari Búsqueda de inhibidores de serina proteasas en glándulas accesorias del sistema reproductor masculino. Reproducción (SAMER) . 2012  
S. D’Ippolito, R. E. De Castro, M.K. Herrera Seitz. Chemotactic responses to gas oil of Halomonas spp strains isolated from saline environments in Argentina Revista Argentina de Microbiología 2011 doi: 10.1590/S0325-75412011000200007
Diego E. Sastre, Roberto A. Paggi and Rosana E. De Castro. The Lon protease from the haloalkaliphilic archaeon Natrialba magadii is transcriptionally linked to a cluster of putative membrane proteases and displays DNA-binding activity Microbiological Research 2011 doi: 10.1016/j.micres.2010.07.002
Bernardini A, Hozbor F, Sanchez E, Fornés MW, Alberio RH and Cesari A. Conserved ram seminal plasma proteins bind to the sperm membrane and repair cryopreservation damage. Theriogenology  2011 doi:10.1016/ j. theriogenology. 2011.02.020
Diego M. Ruiz, Nancy Iannuci, Osvaldo Cascone and Rosana E. De Castro Peptide synthesis catalyzed by a haloalkaliphilic serine protease from the archaeon Natrialba magadii Lett. Appl. Microbiol. 2010 doi: 10.1111/j.1472-765X.2010.02955.x
R. A Paggi, E. A. Madrid, C.P. D’Alessandro, Cerletti, M. and R. E. De Castro Growth phase-dependent biosynthesis of NEP, a halolysin-like protease secreted by the haloalkaliphilic archaeon Natrialba magadii. Lett. Appl.Microbiol. 2010 doi: 10.1111/j.1472-765X.2010.02955.x
Cesari, A, Monclus MA, Tejón GP, Clementi M and Fornes MW. Regulated serine proteinase lytic system on mammalian sperm surface: there must be a role (Review) Theriogenology (2010) 2010 doi: 10.1016/j.theriogenology.2010.03.029
Monclus MA, Andreina C, Cabrillana ME, Saez Lancellotti TE, Rensetti DE, Clementi MA, Boarelli PV, Vincenti AE, Fornés MW. Protein fraction isolated from epididymal fluid re-associates sperm in vitro: Possible role of serpins in rat Rosettes assembly. Molec Reprod Dev.
2010 doi: 10.1002/mrd.21155.
Débora Nercessian, Cristina Marino-Buslje, María Victoria Ordóñez, Rosana E. De Castro and Rubén Danilo Conde Presence of structural homologs to ubiquitin in haloalkaliphilic Archaea Int. Microbiol. 2009 10.2436/20.1501.01.95